Class 12 Biology Molecular Basis of Inhertance: NCERT Solutions for Question 13
What is DNA fingerprinting? Mention its application.
DNA fingerprinting is a technique used to identify and analyze the variations in various individuals at the level of DNA. It is based on variability and polymorphism in DNA sequences.
Application
(1) It is used in forensic science to identify potential crime suspects.
(2) It is used to establish paternity and family relationships.
(3) It is used to identify and protect the commercial varieties of crops and livestock.
(4) It is used to find out the evolutionary history of an organism and trace out the linkages between groups of various organisms.
More Questions From Class 12 Biology - Chapter Molecular Basis of Inhertance
- Q:- Differentiate between the followings:
(a) Repetitive DNA and Satellite DNA
(b) mRNA and tRNA
(c) Template strand and Coding strand - Q:- If the sequence of the coding strand in a transcription unit is written as follows:
5-ATGCATGCATGCATGCATGCATGCATGC-3
Write down the sequence of mRNA. - Q:- How did Hershey and Chase differentiate between DNA and protein in their experiment while proving that DNA is the genetic material?
- Q:- Explain (in one or two lines) the function of the followings:
(a) Promoter
(b) tRNA
(c) Exons - Q:-
Why is the Human Genome project called a mega project?
- Q:- List two essential roles of ribosome during translation.
- Q:- Which property of DNA double helix led Watson and Crick to hypothesise semi-conservative mode of DNA replication? Explain.
- Q:- In the medium where E. coli was growing, lactose was added, which induced the lac operon. Then, why does lac operon shut down some time after addition of lactose in the medium?
- Q:- If the sequence of one strand of DNA is written as follows:
5-ATGCATGCATGCATGCATGCATGCATGC-3
Write down the sequence of complementary strand in 5→3 direction - Q:- If a double stranded DNA has 20 per cent of cytosine, calculate the per cent of adenine in the DNA.
Popular Questions of Class 12 Biology
- Q:- Why is reproduction essential for organisms? Answer
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- Q:-
Fill in the blanks:
(a) Humans reproduce __________. (asexually/sexually)
(b) Humans are__________. (oviparous/viviparous/ovoviviparous)
(c) Fertilization is __________ in humans. (external/internal)
(d) Male and female gametes are __________. (diploid/haploid)
(e) Zygote is __________. (diploid/haploid)
(f) The process of release of the ovum from a mature follicle is called__________.
(g) Ovulation is induced by a hormone called the __________.
(h) The fusion of male and female gametes is called _____________.
(i) Fertilisation takes place in _____________.
(j) Zygote divides to form _____________which is implanted in uterus.
(k) The structure which provides vascular connection between fetus and uterus is called ____________. - Q:- What do you think is the significance of reproductive health in a society?
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- Q:- Bacteria cannot be seen with the naked eyes, but these can be seen with the help of a microscope. If you have to carry a sample from your home to your biology laboratory to demonstrate the presence of microbes under a microscope, which sample would you carry and why?
- Q:- Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).
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- Q:- Why is the offspring formed by asexual reproduction referred to as clone?
- Q:- Correct the following statements:
(a) Surgical methods of contraception prevent gamete formation.
(b) All sexually transmitted diseases are completely curable.
(c) Oral pills are very popular contraceptives among the rural women.
(d) In E. T. techniques, embryos are always transferred into the uterus. - Q:- Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
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(a) Hypocotyl and epicotyl
(b) Coleoptile and coleorrhiza
(c) Integument and testa
(d) Perisperm and pericarp - Q:- Define and design a test − cross?
- Q:- With a neat diagram explain the 7-celled, 8-nucleate nature of the female gametophyte.
- Q:- Identify True/False statements. Correct each false statement to make it true.
(a) Androgens are produced by Sertoli cells. (True/False)
(b) Spermatozoa get nutrition from Sertoli cells. (True/False)
(c) Leydig cells are found in ovary. (True/False)
(d) Leydig cells synthesise androgens. (True/False)
(e) Oogenesis takes place in corpus luteum. (True/False)
(f) Menstrual cycle ceases during pregnancy. (True/False)
(g) Presence or absence of hymen is not a reliable indicator of virginity or sexual experience. (True/False)
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Nice note
DNA fingerprinting is a technique used to identify and analyze the variations in various individuals at the level of DNA. It is based on variability and polymorphism in DNA sequences. DNA Fingerprinting Techniques: 1) Restriction endonuclease analysis of chromosome (REAC) 2) Restriction endonuclease analysis of plasmid DNA (REAP) 3) Restriction fragment length polymorphism (RFLP) 4) PCR-RFLP 5) Random amplified polymorphic DNA (RAPD) 6) Direct amplification fingerprinting (DAF) 7) Repetitive sequence-based PCR (rep-PCR) 8) Pulsed field gel electrophoresis (PFGE) 9) Amplified fragment length polymorphism (AFLP) 10) Single-strand conformational polymorphism (PCRSSCP) 11) Multilocus sequence typing (MLST)
plz answer this question What is DNA fingerprinting? Mention its techniques. best of luck
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