Rhizobium is a symbiotic bacteria present in the root nodules of leguminous plants. The basic requirements for Rhizobium to carry out nitrogen fixation are as follows:
(a) Presence of the enzyme nitrogenase
(b) Presence of leg-haemoglobin
(c) Non-haem iron protein, ferrodoxin as the electron-carrier
(d) Constant supply of ATP
(e) Mg2+ions as co-factors
Rhizobium contains the enzyme nitrogenase – a Mo-Fe protein – that helps in the conversion of atmospheric free nitrogen into ammonia.
The reaction is as follows:
N2 + 8e– + 8H+ + 16 ATP→ 2 NH3 + H2 + 16ADP + 16Pi
The Rhizobium bacteria live as aerobes under free-living conditions, but require anaerobic conditions during nitrogen fixation. This is because the enzyme nitrogenase is highly sensitive to molecular oxygen. The nodules contain leg-haemoglobin, which protects nitrogenase from oxygen.
What are the steps involved in formation of a root nodule?
Which of the following is not correct?
(a) Robert Brown discovered the cell.
(b) Schleiden and Schwann formulated the cell theory.
(c) Virchow explained that cells are formed from pre-existing cells.
(d) A unicellular organism carries out its life activities within a single cell.
Bile juice contains no digestive enzymes, yet it is important for digestion. Why?
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