Class 12 Biology - Chapter Biotechnology Principles and Process NCERT Solutions | Can you think and answer how a reporter
Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
A reporter gene can be used to monitor the transformation of host cells by foreign DNA. They act as a selectable marker to determine whether the host cell has taken up the foreign DNA or the foreign gene gets expressed in the cell. The researchers place the reporter gene and the foreign gene in the same DNA construct. Then, this combined DNA construct is inserted in the cell. Here, the reporter gene is used as a selectable marker to find out the successful uptake of genes of interest (foreign genes). An example of reporter genes includes lac Z gene, which encodes a green fluorescent protein in a jelly fish.
More Questions From Class 12 Biology - Chapter Biotechnology Principles and Process
- Q:- Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).
- Q:- What would be the molar concentration of human DNA in a human cell? Consult your teacher.
- Q:- Do eukaryotic cells have restriction endonucleases? Justify your answer.
- Q:- Describe briefly the followings:
(a) Origin of replication
(b) Bioreactors
(c) Downstream processing - Q:- Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?
- Q:- Explain briefly
(a) PCR
(b) Restriction enzymes and DNA
(c) Chitinase - Q:- From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?
- Q:- Discuss with your teacher and find out how to distinguish between
(a) Plasmid DNA and chromosomal DNA
(b) RNA and DNA
(c) Exonuclease and Endonuclease - Q:- Collect 5 examples of palindromic DNA sequences by consulting your teacher. Better try to create a palindromic sequence by following base-pair rules.
- Q:- Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
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Fill in the blanks:
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1 Comment(s) on this Question
Thanks a lot, it's really gud answer
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