Class 12th Biology 2016 Set3 Delhi Board Paper Solution
Question 12
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
Answer
PCR : Polymerase Chain Reaction.
Multiple copies of the gene of interest is synthesised in vitro using two sets of primers and enzyme DNA polymerase. The enzyme extends the primers using nucleotides provided and genomic DNA as template. The process of DNA replication is repeated several times for amplification of DNA with the help of thermostable DNA polymerase which remains active during high temperature induced denaturation of double stranded DNA.
Popular Questions of Class 12th biology
- Q:- What is spermatogenesis? Briefly describe the process of spermatogenesis.
- Q:- Why is reproduction essential for organisms? Answer
- Q:- Write a short note on
(a) Adaptations of desert plants and animals
(b) Adaptations of plants to water scarcity
(c) Behavioural adaptations in animals
(d) Importance of light to plants
(e) Effect of temperature or water scarcity and the adaptations of animals. - Q:- With a neat diagram explain the 7-celled, 8-nucleate nature of the female gametophyte.
- Q:- What is DNA fingerprinting? Mention its application.
- Q:- What is oogenesis? Give a brief account of oogenesis.
- Q:- Describe the structure of a seminiferous tubule.
- Q:- What is triple fusion? Where and how does it take place? Name the nuclei involved in triple fusion.
- Q:- With a neat, labelled diagram, describe the parts of a typical angiosperm ovule.
- Q:- Explain the Law of Dominance using a monohybrid cross.
Recently Viewed Questions of Class 12th biology
- Q:- If your family owned a dairy farm, what measures would you undertake to improve the quality and quantity of milk production?
- Q:- How does the transmission of each of the following diseases take place?
(a) Amoebiasis
(b) Malaria
(c) Ascariasis
(d) Pneumonia - Q:- What are the major functions of male accessory ducts and glands?
- Q:- Define ecological pyramids and describe with examples, pyramids of number and biomass.
- Q:- Explain what is meant by metastasis.
- Q:- Name the primary and secondary lymphoid organs.
- Q:- In which food would you find lactic acid bacteria? Mention some of their useful applications.
- Q:- Offspring formed due to sexual reproduction have better chances of survival. Why? Is this statement always true?
- Q:- List the various abiotic environmental factors.
- Q:- Define decomposition and describe the processes and products of decomposition.