Class 12th Biology 2013 Set2 Delhi Board Paper Solution
Question 10
Expand the following and mention one application of each:
Expand the following and mention one application of each:
(i) PCR
(ii) ELISA
OR (a) Mention the difference in the mode of action of exonuclease and endonuclease.
(b) How does restriction endonuclease function?
Answer
(i) PCR - Polymerase Chain Reaction
(ii) ELISA - Enzyme linked Immuno sorbent Assay.
OR
(a) Exonuclease - cuts the DNA at free ends.
Endonuclease - cuts within DNA segment
(b) Resticriction endonuclease, : cuts within the DNA, at specific sites called pallindromic sites.
e.g. Eco R1 cuts the DNA at following pallindromic sequences.
|
5' G A A T T C 3'
3' C T T A A G 5'
|
Popular Questions of Class 12th biology
- Q:- What is spermatogenesis? Briefly describe the process of spermatogenesis.
- Q:- Why is reproduction essential for organisms? Answer
- Q:- Write a short note on
(a) Adaptations of desert plants and animals
(b) Adaptations of plants to water scarcity
(c) Behavioural adaptations in animals
(d) Importance of light to plants
(e) Effect of temperature or water scarcity and the adaptations of animals. - Q:- With a neat diagram explain the 7-celled, 8-nucleate nature of the female gametophyte.
- Q:- What is DNA fingerprinting? Mention its application.
- Q:- What is oogenesis? Give a brief account of oogenesis.
- Q:- Describe the structure of a seminiferous tubule.
- Q:- What is triple fusion? Where and how does it take place? Name the nuclei involved in triple fusion.
- Q:- With a neat, labelled diagram, describe the parts of a typical angiosperm ovule.
- Q:- Explain the Law of Dominance using a monohybrid cross.
Recently Viewed Questions of Class 12th biology
- Q:- What are the various routes by which transmission of human immunodeficiency virus takes place?
- Q:- Identify each part in a flowering plant and write whether it is haploid (n) or diploid (2n).
(a) Ovary _______________
(b) Anther _______________
(c) Egg _________________
(d) Pollen__________________
(e) Male gamete _______________
(f ) Zygote ___________________ - Q:- Explain why meiosis and gametogenesis are always interlinked?
- Q:- Briefly describe the following:
(a) Transcription
(b) Polymorphism
(c) Translation
(d) Bioinformatics - Q:- Can you suggest a method to remove oil (hydrocarbon) from seeds based on your understanding of rDNA technology and chemistry of oil?
- Q:- Differentiate between gametogenesis from embryogenesis.
- Q:- List two essential roles of ribosome during translation.
- Q:- Why is reproduction essential for organisms? Answer
- Q:- If the sequence of one strand of DNA is written as follows:
5-ATGCATGCATGCATGCATGCATGCATGC-3
Write down the sequence of complementary strand in 5→3 direction - Q:- What is apiculture? How is it important in our lives?