Explain the roles of the following with the help of an example each in recombinant DNA technology:
(a) Restriction enzymes
(b) Plasmids
(a) Restriction enzymes: These are the molecular scissor that cut the DNA strand into fragment. The type of reotriction enzymes are: Endonuclease and Exonuclease.
Endonuclease cut the DNA sequence at the specific site where as exonuclease cut the DNA strands at the end.
(b) Plasmids: Plasmids are the double stranded circular DNA other than chromosomal DNA present in prokaryotes. Plasmids are used as vector in DNA technology because these are responsible to determine the phenotypic character.
Fill in the blanks:
(a) Humans reproduce __________. (asexually/sexually)
(b) Humans are__________. (oviparous/viviparous/ovoviviparous)
(c) Fertilization is __________ in humans. (external/internal)
(d) Male and female gametes are __________. (diploid/haploid)
(e) Zygote is __________. (diploid/haploid)
(f) The process of release of the ovum from a mature follicle is called__________.
(g) Ovulation is induced by a hormone called the __________.
(h) The fusion of male and female gametes is called _____________.
(i) Fertilisation takes place in _____________.
(j) Zygote divides to form _____________which is implanted in uterus.
(k) The structure which provides vascular connection between fetus and uterus is called ____________.